The action or process of introducing DNA or chromosomes into bacteria or other cells using a pulse of electricity to briefly open the pores in the cell membranes.
- DNA was introduced into DH10ß cells via electroporation with a Gene Pulser II (Bio-Rad).
- Plasmids pJF138 and pJF141 were introduced into cells by electroporation.
- In molecular biology, the process of electroporation is often used for the transformation of bacteria, yeast, and plant protoplasts.
- Example sentences
- Plasmid DNA was electroporated into 60 l of electrocompetent Escherichia coli C and incubated in a shaker/incubator for 30 min at 37°C for the positive selection of mutants.
- This plasmid was electroporated into Agrobacterium tumefaciens strain GV3101, which was used to transform pex5-1 mutant plants by the floral dip method.
- Early observations that poly tails can enhance expression of reporter mRNAs electroporated into plant protoplasts were interpreted as evidence for the role of poly tails in translation in plants.
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