The plane through the focus perpendicular to the axis of a mirror or lens.
- Then, the cell- and bead-holding pipettes were carefully aligned to get the focal plane and the plane of cell-bead contact to coincide.
- A lens focuses this light at the back focal plane of the objective to allow collimation at the sample.
- This setup captures only light from the focal plane, while out-of-focus Raman light and fluorescence background is strongly rejected.
Definition of focal plane in:
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