(also restriction endonuclease)
An enzyme produced chiefly by certain bacteria, having the property of cleaving DNA molecules at or near a specific sequence of bases.
- The term restriction endonuclease was given to this class of bacterially derived enzymes since they were identified as being involved in restricting the growth of certain bacteriophages.
- The cDNA is digested with a restriction enzyme (anchoring enzyme) that cleaves the cDNA into fragments of approximately 256 base pairs.
- The presence of a particular mutation may create or destroy a restriction site, and the DNA fragments produced following restriction enzyme digestion show characteristic banding patterns when electrophoretically separated.
Definition of restriction enzyme in:
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