The artificial insertion of random fragments of DNA from a donor organism into a recipient by genetic engineering.
- In science and technology, the fast-moving field of genetics has given us some colourful new terms: it's a world of pathogenicity islands, shotgun cloning and terminator genes.
- Microbial diversity and community structure in soil samples from conventional and organic farming sites were analysed using denaturing gradient gel electrophoresis and shotgun cloning.
- We used it at several steps in our cloning procedure including recovery of the genomic fragments for shotgun cloning and eventual sequencing.
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