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sonicate

Line breaks: son¦ic|ate
Pronunciation: /ˈsɒnɪkeɪt
 
/
Biochemistry

Definition of sonicate in English:

verb

[with object]
Subject (a biological sample) to ultrasonic vibration so as to fragment the cells, macromolecules, and membranes: cells were sonicated in an ice bath
More example sentences
  • The sample was sonicated to clarity in a sonication bath (Ultrawave).
  • The samples were sonicated for 30 min, followed by centrifugation and overnight incubation at 65°C as described above.
  • The samples were sonicated for 12 sec and cell number was analyzed using a Coulter counter.

noun

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A biological sample which has been sonicated.
Example sentences
  • Finally, the sonicate in the third cuvette was additionally treated with DNase for another 1 h at 25 deg C, then Cyan 40 was added and the emission spectrum was recorded.
  • The sonicate was cleared by centrifugation at 12,000 g for 30 minutes at 4°C.
  • The sonicate was cleared by microcentrifugation for 5 min at 4° and mixed with the anti-HA.11 antibody coupled to Sepharose beads (prewashed three times with lysis buffer).

Origin

1950s: from sonic + -ate2.

Derivatives

sonication

1
noun
Example sentences
  • Lipid hydration was coupled with six cycles of freezing, thawing, and sonication, except sonication was omitted in the last cycle.
  • Chromatin was sheared by sonication, and DNA associated with either CLF1 or ORC1 was separately co-immunoprecipitated using an anti-HA antibody.
  • Cells were disrupted by sonication, and soluble recombinant proteins were purified on the TALON metal-affinity resin (Clontech).

Definition of sonicate in:

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