An enzyme that removes successive nucleotides from the end of a polynucleotide molecule.
- Orn was shown by in vitro analysis to be an exonuclease specific for RNA molecules shorter than five nucleotides in length.
- A given exonuclease hydrolyzes DNA at a fixed rate, but there exist a distribution of conformations and a distribution of hydrolysis rates.
- The rapid rise of the first fluorescence maximum puts a strict upper bound on the timescale of magnesium binding to the exonuclease.
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