A topologically distinct isomer, especially of DNA.
- The distribution of 2 plasmid DNA topoisomers was measured by Southern blot analysis with a radiolabeled 2 DNA probe.
- Comparing the topoisomer distribution of plasmid DNA from the ancestor with the completely relaxed plasmid control yielded an estimate of = - 0.066 for the ancestor.
- An extract was deemed to be deficient if, in all three trials, the ratio of intermediate to supercoiled products indicated an accumulation of intermediate topoisomers (compared to wild type).
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